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y2h gold yeast strain  (TaKaRa)


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    Structured Review

    TaKaRa y2h gold yeast strain
    All proteins are from the bread wheat Chinese Spring genome. A ) Yeast two-hybrid <t>(Y2H)</t> assay showing interactions between AD: Ta MFP1.1 or AD: Ta MFP1.2 and BD: Ta BGC1, where AD is the activation domain and BD is the binding domain. Cells were plated on double dropout medium (DDO; -Leu-Trp) to analyse growth and quadruple dropout medium (QDO; -Ade-Leu-Trp-His) to analyse the interaction. PC, positive control (T-antigen and p53); NC, negative control (T-antigen and lamin). B ) Pairwise immunoprecipitation (IP) of Ta MFP1.1-YFP or Ta MFP1.2-YFP and Ta BGC1-RFP co-expressed in N. benthamiana leaves, using anti-YFP beads. Blots of input and IP samples were probed with YFP (top panels) and RFP antibodies (bottom panels). Chloroplast-targeted YFP (cTP-YFP) and RFP (cTP-RFP) were used as controls to exclude non-specific binding to the fluorescent protein tags.
    Y2h Gold Yeast Strain, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/y2h+gold+yeast+strain/Y2HGold+Yeast+Strain/bio_rxiv__64898__2026__04__21__719982-196-7-11
    Average 96 stars, based on 1592 article reviews
    y2h gold yeast strain - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Two redundant paralogs of MAR1-BINDING FILAMENT PROTEIN affect B-type granule initiation in wheat"

    Article Title: Two redundant paralogs of MAR1-BINDING FILAMENT PROTEIN affect B-type granule initiation in wheat

    Journal: bioRxiv

    doi: 10.64898/2026.04.21.719982

    All proteins are from the bread wheat Chinese Spring genome. A ) Yeast two-hybrid (Y2H) assay showing interactions between AD: Ta MFP1.1 or AD: Ta MFP1.2 and BD: Ta BGC1, where AD is the activation domain and BD is the binding domain. Cells were plated on double dropout medium (DDO; -Leu-Trp) to analyse growth and quadruple dropout medium (QDO; -Ade-Leu-Trp-His) to analyse the interaction. PC, positive control (T-antigen and p53); NC, negative control (T-antigen and lamin). B ) Pairwise immunoprecipitation (IP) of Ta MFP1.1-YFP or Ta MFP1.2-YFP and Ta BGC1-RFP co-expressed in N. benthamiana leaves, using anti-YFP beads. Blots of input and IP samples were probed with YFP (top panels) and RFP antibodies (bottom panels). Chloroplast-targeted YFP (cTP-YFP) and RFP (cTP-RFP) were used as controls to exclude non-specific binding to the fluorescent protein tags.
    Figure Legend Snippet: All proteins are from the bread wheat Chinese Spring genome. A ) Yeast two-hybrid (Y2H) assay showing interactions between AD: Ta MFP1.1 or AD: Ta MFP1.2 and BD: Ta BGC1, where AD is the activation domain and BD is the binding domain. Cells were plated on double dropout medium (DDO; -Leu-Trp) to analyse growth and quadruple dropout medium (QDO; -Ade-Leu-Trp-His) to analyse the interaction. PC, positive control (T-antigen and p53); NC, negative control (T-antigen and lamin). B ) Pairwise immunoprecipitation (IP) of Ta MFP1.1-YFP or Ta MFP1.2-YFP and Ta BGC1-RFP co-expressed in N. benthamiana leaves, using anti-YFP beads. Blots of input and IP samples were probed with YFP (top panels) and RFP antibodies (bottom panels). Chloroplast-targeted YFP (cTP-YFP) and RFP (cTP-RFP) were used as controls to exclude non-specific binding to the fluorescent protein tags.

    Techniques Used: Y2H Assay, Activation Assay, Binding Assay, Positive Control, Negative Control, Immunoprecipitation

    Related Articles

    Construct:

    Article Title: Two redundant paralogs of MAR1-BINDING FILAMENT PROTEIN affect B-type granule initiation in wheat
    Article Snippet: .. For Y2H, constructs were transformed into the Y2H Gold yeast strain (Clontech) using the EZ-Yeast Transformation Kit (MP Biomedicals) according to the manufacturer’s protocol. ..

    Article Title: Exploring Virus–Host Interactions Through Combined Proteomic Approaches Identifies BANF1 as a New Essential Factor for African Swine Fever Virus
    Article Snippet: ORFs encoding A151R and MGF360-21R were fused with a Gal4 DNA-binding domain (Gal4-BD), within the pDEST32 vector (Invitrogen). .. These constructs were transformed into the Y2H Gold yeast strain (Clontech) and selected on a selective medium lacking leucine (-L). .. Y2H screens were performed using a swine cDNA library, which was generated with mRNAs extracted from PAM and then cloned into the Gal4 transactivation domain (Gal4-AD) pDEST22 vector (Creative Biogene).

    Article Title: SbHMA5 is a P1B-type Cu ATPase involved in Cu homeostasis by interacting with metallochaperones SbATX1 and SbFRN3.
    Article Snippet: Copper (Cu) is required by prokaryotes and eukaryotes for fundamental life processes including photosynthesis, respiration and neurotransmission but excess Cu is toxic, causing severe problems ranging from weak plant growth to Wilson’s disease in humans.. Copper homeostasis was shown to be tightly regulated by P1B-type ATPase in yeast and other model organisms but the molecular mechanism by which sorghum plants maintain this delicate balance of Cu homeostasis remains poorly understood.. We identified 31 orthologs of P1B-type metal transporters in Sorghum bicolor.

    Article Title: HPE21, a liberibacter effector modulating H2O2 accumulation in tomato plants
    Article Snippet: .. The desired pair of bait and prey constructs were co-transformed into Y2H Gold yeast strain and plated on double dropout medium (SD-Leu-Trp, DDO) (Takara Bio, # 630317). ..

    Transformation Assay:

    Article Title: Two redundant paralogs of MAR1-BINDING FILAMENT PROTEIN affect B-type granule initiation in wheat
    Article Snippet: .. For Y2H, constructs were transformed into the Y2H Gold yeast strain (Clontech) using the EZ-Yeast Transformation Kit (MP Biomedicals) according to the manufacturer’s protocol. ..

    Article Title: Exploring Virus–Host Interactions Through Combined Proteomic Approaches Identifies BANF1 as a New Essential Factor for African Swine Fever Virus
    Article Snippet: ORFs encoding A151R and MGF360-21R were fused with a Gal4 DNA-binding domain (Gal4-BD), within the pDEST32 vector (Invitrogen). .. These constructs were transformed into the Y2H Gold yeast strain (Clontech) and selected on a selective medium lacking leucine (-L). .. Y2H screens were performed using a swine cDNA library, which was generated with mRNAs extracted from PAM and then cloned into the Gal4 transactivation domain (Gal4-AD) pDEST22 vector (Creative Biogene).

    Article Title: Overexpression of BcPIF4-1 affects flowering time and hypocotyl length by regulating AtFT and AtIAA29 expression, respectively, in Arabidopsis thaliana.
    Article Snippet: Abstract PHYTOCHROME INTERACTING FACTOR4 (PIF4) plays an important role in regulating plant thermomorphogenesis.. In this study, two PIF4 homologous genes, BcPIF4-1 and BcPIF4-2 (Brassica rapa subsp. CHINENSIS PIF4-1 and PIF4-2), were investigated.. Amino acid sequence comparison with Arabidopsis thaliana PIF4 (AtPIF4) showed that BcPIF4-1 and BcPIF4-2 had an active phytochrome B binding (APB) motif at the N-terminus and a basic helix–loop–helix (bHLH) domain at the C-terminus.

    Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk
    Article Snippet: .. Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Y187 yeast strain was purchased from Clontech (Cat. 630482).

    Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk.
    Article Snippet: .. Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Y187 yeast strain was purchased from Clontech (Cat. 630482).

    Sequencing:

    Article Title: SbHMA5 is a P1B-type Cu ATPase involved in Cu homeostasis by interacting with metallochaperones SbATX1 and SbFRN3.
    Article Snippet: Copper (Cu) is required by prokaryotes and eukaryotes for fundamental life processes including photosynthesis, respiration and neurotransmission but excess Cu is toxic, causing severe problems ranging from weak plant growth to Wilson’s disease in humans.. Copper homeostasis was shown to be tightly regulated by P1B-type ATPase in yeast and other model organisms but the molecular mechanism by which sorghum plants maintain this delicate balance of Cu homeostasis remains poorly understood.. We identified 31 orthologs of P1B-type metal transporters in Sorghum bicolor.

    Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk
    Article Snippet: .. Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Y187 yeast strain was purchased from Clontech (Cat. 630482).

    Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk.
    Article Snippet: .. Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Y187 yeast strain was purchased from Clontech (Cat. 630482).

    Clone Assay:

    Article Title: SbHMA5 is a P1B-type Cu ATPase involved in Cu homeostasis by interacting with metallochaperones SbATX1 and SbFRN3.
    Article Snippet: Copper (Cu) is required by prokaryotes and eukaryotes for fundamental life processes including photosynthesis, respiration and neurotransmission but excess Cu is toxic, causing severe problems ranging from weak plant growth to Wilson’s disease in humans.. Copper homeostasis was shown to be tightly regulated by P1B-type ATPase in yeast and other model organisms but the molecular mechanism by which sorghum plants maintain this delicate balance of Cu homeostasis remains poorly understood.. We identified 31 orthologs of P1B-type metal transporters in Sorghum bicolor.

    Plasmid Preparation:

    Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk
    Article Snippet: .. Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Y187 yeast strain was purchased from Clontech (Cat. 630482).

    Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk.
    Article Snippet: .. Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Y187 yeast strain was purchased from Clontech (Cat. 630482).



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    All proteins are from the bread wheat Chinese Spring genome. A ) Yeast two-hybrid <t>(Y2H)</t> assay showing interactions between AD: Ta MFP1.1 or AD: Ta MFP1.2 and BD: Ta BGC1, where AD is the activation domain and BD is the binding domain. Cells were plated on double dropout medium (DDO; -Leu-Trp) to analyse growth and quadruple dropout medium (QDO; -Ade-Leu-Trp-His) to analyse the interaction. PC, positive control (T-antigen and p53); NC, negative control (T-antigen and lamin). B ) Pairwise immunoprecipitation (IP) of Ta MFP1.1-YFP or Ta MFP1.2-YFP and Ta BGC1-RFP co-expressed in N. benthamiana leaves, using anti-YFP beads. Blots of input and IP samples were probed with YFP (top panels) and RFP antibodies (bottom panels). Chloroplast-targeted YFP (cTP-YFP) and RFP (cTP-RFP) were used as controls to exclude non-specific binding to the fluorescent protein tags.
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    Image Search Results


    All proteins are from the bread wheat Chinese Spring genome. A ) Yeast two-hybrid (Y2H) assay showing interactions between AD: Ta MFP1.1 or AD: Ta MFP1.2 and BD: Ta BGC1, where AD is the activation domain and BD is the binding domain. Cells were plated on double dropout medium (DDO; -Leu-Trp) to analyse growth and quadruple dropout medium (QDO; -Ade-Leu-Trp-His) to analyse the interaction. PC, positive control (T-antigen and p53); NC, negative control (T-antigen and lamin). B ) Pairwise immunoprecipitation (IP) of Ta MFP1.1-YFP or Ta MFP1.2-YFP and Ta BGC1-RFP co-expressed in N. benthamiana leaves, using anti-YFP beads. Blots of input and IP samples were probed with YFP (top panels) and RFP antibodies (bottom panels). Chloroplast-targeted YFP (cTP-YFP) and RFP (cTP-RFP) were used as controls to exclude non-specific binding to the fluorescent protein tags.

    Journal: bioRxiv

    Article Title: Two redundant paralogs of MAR1-BINDING FILAMENT PROTEIN affect B-type granule initiation in wheat

    doi: 10.64898/2026.04.21.719982

    Figure Lengend Snippet: All proteins are from the bread wheat Chinese Spring genome. A ) Yeast two-hybrid (Y2H) assay showing interactions between AD: Ta MFP1.1 or AD: Ta MFP1.2 and BD: Ta BGC1, where AD is the activation domain and BD is the binding domain. Cells were plated on double dropout medium (DDO; -Leu-Trp) to analyse growth and quadruple dropout medium (QDO; -Ade-Leu-Trp-His) to analyse the interaction. PC, positive control (T-antigen and p53); NC, negative control (T-antigen and lamin). B ) Pairwise immunoprecipitation (IP) of Ta MFP1.1-YFP or Ta MFP1.2-YFP and Ta BGC1-RFP co-expressed in N. benthamiana leaves, using anti-YFP beads. Blots of input and IP samples were probed with YFP (top panels) and RFP antibodies (bottom panels). Chloroplast-targeted YFP (cTP-YFP) and RFP (cTP-RFP) were used as controls to exclude non-specific binding to the fluorescent protein tags.

    Article Snippet: For Y2H, constructs were transformed into the Y2H Gold yeast strain (Clontech) using the EZ-Yeast Transformation Kit (MP Biomedicals) according to the manufacturer’s protocol.

    Techniques: Y2H Assay, Activation Assay, Binding Assay, Positive Control, Negative Control, Immunoprecipitation