y2h gold yeast strain (TaKaRa)
Structured Review

Y2h Gold Yeast Strain, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y2h+gold+yeast+strain/Y2HGold+Yeast+Strain/bio_rxiv__64898__2026__04__21__719982-196-7-11
Average 96 stars, based on 1592 article reviews
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1) Product Images from "Two redundant paralogs of MAR1-BINDING FILAMENT PROTEIN affect B-type granule initiation in wheat"
Article Title: Two redundant paralogs of MAR1-BINDING FILAMENT PROTEIN affect B-type granule initiation in wheat
Journal: bioRxiv
doi: 10.64898/2026.04.21.719982
Figure Legend Snippet: All proteins are from the bread wheat Chinese Spring genome. A ) Yeast two-hybrid (Y2H) assay showing interactions between AD: Ta MFP1.1 or AD: Ta MFP1.2 and BD: Ta BGC1, where AD is the activation domain and BD is the binding domain. Cells were plated on double dropout medium (DDO; -Leu-Trp) to analyse growth and quadruple dropout medium (QDO; -Ade-Leu-Trp-His) to analyse the interaction. PC, positive control (T-antigen and p53); NC, negative control (T-antigen and lamin). B ) Pairwise immunoprecipitation (IP) of Ta MFP1.1-YFP or Ta MFP1.2-YFP and Ta BGC1-RFP co-expressed in N. benthamiana leaves, using anti-YFP beads. Blots of input and IP samples were probed with YFP (top panels) and RFP antibodies (bottom panels). Chloroplast-targeted YFP (cTP-YFP) and RFP (cTP-RFP) were used as controls to exclude non-specific binding to the fluorescent protein tags.
Techniques Used: Y2H Assay, Activation Assay, Binding Assay, Positive Control, Negative Control, Immunoprecipitation
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